Blood Test For Pregnancy
26.03.2016 Andrew Johns 0 Popular
Determine the Pregnancy By a Blood Test
A pregnancy blood test is based on identifying the level of “pregnancy hormone” in blood. This special pregnancy hormone is human chorionic gonadotropin (hCG), which is produced by the chorion, a membrane that covers the embryo. The presence of chorionic villi in the blood means a pregnancy. That makes an early pregnancy blood test possible: you can get the positive result in one week after the conceiving. Usually, the hCG blood level of a woman who is not pregnant is about 0-15 mIU/ml.
The hCG test is also performed to find any pathology in the pregnancy (weeks 14-18). You can take an accurate hCG test at any time convenient for you (express result available) in the Traditional Midwifery and Family Care Centre.
How the HCG Test is Performed
Traditionally, a pregnancy blood test is performed on the 8th day after conceiving, but not earlier than 3-5 days of period delay. The blood is drawn from the arm vein on an empty stomach in the morning. If you can’t do the blood test in the morning, just remember that you have to fast for 4-6 hours before the test. Please inform the doctor or the nurse if you have been taking any drugs or hormonal medications. You can take another test in a couple of days to receive even more accurate results.
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- Blood rapid pregnancy test
- Convenience: While it is true that a quantitative blood test can confirm pregnancy a couple of days before an early detection HPT, a qualitative blood test that is set to detect 25 mIU/ml is no more sensitive than an early detection HPT. Many women find it more convenient to simply wait a day or two and take a pregnancy test in the privacy of their home. In addition, an HPT will typically produce results in just minutes. On the other hand, you might be on pins and needles for up to 48 hours while you wait for results from a blood pregnancy test.
- Expense: The out of pocket expense for a blood pregnancy test will vary depending on the clinic you choose and the type of insurance you have. You should expect to pay up to about $75 for this service. And, if the results are uncertain (hCG level between 5 mIU/ml and 25 mIU/ml), a retest will be required in a few days, which will in most cases incur an additional fee. On the other hand, you can purchase 10 early detection pregnancy test strips for about $10, which will allow you to test several days in a row if necessary.
- Accuracy: The most “accurate” pregnancy test is the quantitative blood pregnancy test, as it determines the exact amount of hCG in your blood. This will allow your doctor to make a very clear determination about whether or not you are pregnant or if a retest is required in a few more days. With both a qualitative blood test and an HPT it is possible to get a false negative result. This means that if the test is not sensitive enough to detect your current level of hCG (for example, your current level is 20 mIU and the test is set to detect hCG at 25 mIU), you will get a negative test result even though you are pregnant. If you get a negative result from an HPT or a qualitative blood test, and you continue to have any signs of pregnancy, we recommend retesting in a few days.
- Fertility history: If you have experienced a miscarriage or an ectopic pregnancy in the past, your doctor may well want to monitor your hCG levels closely in the first several weeks of pregnancy. In the early days of pregnancy, hCG levels double every 48-72 hours. (See the chart below for average blood hCG levels as pregnancy advances). If hCG levels do not rise appropriately in those very early days, it can signal a problem with the pregnancy. By monitoring your exact hCG levels with regular quantitative blood test, your doctor may be able to detect a problem early on. Neither a qualitative blood test nor an HPT allows this type of monitoring, as they do not reveal exact amounts of hCG, and cannot determine if levels are increasing appropriately.
- 3 weeks since LMP: 5 – 50 mIU/ml
- 4 weeks since LMP: 5 – 426 mIU/ml
- 5 weeks since LMP: 18 – 7,340 mIU/ml
- 6 weeks since LMP: 1,080 – 56,500 mIU/ml
- 7 – 8 weeks since LMP: 7,650 – 229,000 mIU/ml
- 9 – 12 weeks since LMP: 25,700 – 288,000 mIU/ml
- 13 – 16 weeks since LMP: 13,300 – 254,000 mIU/ml
- 17 – 24 weeks since LMP: 4,060 – 165,400 mIU/ml
- 25 – 40 weeks since LMP: 3,640 – 117,000 mIU/ml
Blood rapid pregnancy test
Blood rapid pregnancy test
The NG-Test hCG WB is a rapid visual immunoassay for the qualitative presumptive detection of human chorionic gonadotropin in human whole blood to aid in the early detection of pregnancy.The NG-Test hCG WB has a sensitivity of 10mIU/mL
Human chorionic gonadotropin (hCG) is a glycoprotein secreted by viable placenta tissue during pregnancy. In normal pregnancy,hCG can be detected in whole blood as early as 7 to 10 days after conception. hCG levels continue to rise very rapidly, frequently exceeding 100 mIU/mL make it an excellent marker for confirming pregnancy. The test utilizes monoclonal antibodies to hCG.
The NG-Test hCG WB is a rapid chromatographic immunoassay for the qualitative detection of Human Chorionic gonadotropin (hCG) in whole blood. The test utilizes monoclonal antibodies to hCG to selectively detect elevated levels of hCG in whole blood. The assay is conducted dispensing an adequate volume of the blood specimen into the sample well of the cassette. A buffer solution is added to the well to facilitate the reagent capillary action across the strip held in the cassette. The sample then migrates across a membrane toward the results window where the labeled hCG complex is captured at a test line region containing immobilized monoclonal anti-hCG. Control region, whatever the result should show a colored band. It indicates that the test has been performed correctly. The appearance of two red lines, one at T (Test) and the other at C (Control) indicates the presence of hCG in the sample. If a detectable level of Hcg (<10 mIU/ml) is not present, only the control band will appear in the result window.
Reagents and material provided
Each kit contains 10 individual pouches containing:
• 1 cassette, each sealed in a foil pouch with dessiccant.
• 1 calibrated micropipettes capable delivering 20µl accurately.
• 1 alcohol prep pad.
• 1 Buffer solution in plastic dropper bottle.
• 2 traceability patient labels.
• 1 package insert.
Material required but not provided
• For Professional in vitro diagnostic use only.
• Do not use after the expiration date.
• The test device should remain in the sealed pouches until use.
• Handle all specimens as potentially infectious.
• The test device should be discarded in a suitable biohazardous waste container after testing.
• The test device should not be reused.
Store as packaged in the sealed pouch at 4-30°C.Test devices are stable until the expiration date printed on the kit or foil pouch. DO NOT FREEZE.
Fresh blood from finger prick / puncture should be used as a test specimen. However, fresh anti coagulated whole blood may also be used as a test sample and EDTA or Heparin or Oxalate can be used as suitable anticoagulant. The specimen should be collected in a clean glass or plastic container. If immediate testing is not possible then the specimen may be stored at 2-8°C for up to 72 hours before testing. Clotted or contaminated blood samples should not be used for performing the test.
1. Wear protective gloves.
2. Bring the kit components to room temperature before testing.
3. In case the pouch has been stored at 4-8°C, allow at least 10 minutes for the device to come to room temperature.
4. Open the pouch and remove the device. Once opened, the device must be used immediately.
5. For easy blood sample collection the patient should maintain the arm hang down for at least 3 minutes. Cleanse with alcohol swab the area of the 3rd or 4th finger of the left hand and let dry before pricking.
6. Open the lancet and prick the lateral side of the patient’s fingertip to obtain a drop of blood (20μl). Using gentle pressure, massage the finger towards the fingertip to encourage a drop of blood to form.
7. Hold the capillary micropipette horizontaly, and touch with the tip of the micropipette the blood sample.
CAUTION: Filling is automatic by capilary action. Do not squeeze the tube while sampling.
8. To expel the sample, place the tip of the micropipette on the Sample pad hole marked “S” and squeeze the micropipette bulb. Transfer to the sample pad must be immediate in order to avoid sample clotting.
9. Add 2 drops of buffer solution (2×40µl) into the Reagent hole marked “R”.
10. Read the results at 5 minutes and interprete as below.
NOTE: Do not interprete the test results after 10 minutes. (Results may change.)
If there is only one red color band in the control region (C), this indicates that the specimen does not contain a detectable level of hCG and should be interpreted as a negative result
If there are two red color bands one in the control region (C) and another in the test region (T), this indicates that the specimen contains hCG at a concentration equal to or greater than 10 mIU/mL and should be interpreted as positive result.
If the control band will not appear the test result is invalid. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Deterioration of the test kit may have occurred. Repeat the test procedure using a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.
NOTE: The intensity of the red color in the test line region (T) will vary depending on the concentration of hCG present in the specimen. However, neither the quantitative value nor the rate of increase in hCG can be determined by this qualitative test2.
Internal procedural controls are included in the test. The appearance of the control band confirms sufficient specimen volume and correct procedural technique.
At the read time, a clear background is an internal negative background control. If the test working properly, the background in the result area should be white to light pink and not to interfere with the ability to read the test result.
It is recommended that external hCG controls be run with each new lot.
1. False negative results may occur when the levels of hCG are below the sensitivity level of the test (10mIU/mL). When pregnancy is still suspected repeat the test 48 hours later.
2. Very low levels of hCG are present in whole blood specimen shortly after implantation. However, because a significant number of first trimester pregnancies terminate for natural reasons 5, a test result weakly positive should be confirmed by retesting 48 hours later.
3. A number of conditions other than pregnancy, including trophoblastic disease, testicular tumors, prostate cancer, breast cancer and lung cancer cause elevated levels of hCG 6-7. Therefore, the presence of hCG in whole blood specimen should not be used to diagnose pregnancy unless these conditions have been ruled out.
4. As with any assay employing mouse antibodies, the possibility exists for interferences by human anti-mouse antibodies (HAMA) in the specimen. Specimens from patients who have received preparations of monoclonal antibodies for diagnosis therapy may contain HAMA. Such specimens may cause false positive or false negative results.
5. This test provides a presumptive diagnosis for pregnancy.
A confirmed pregnancy diagnosis should be made by a physician after all clinical and laboratory findings have been evaluated.
Negative results are expected in healthy non-pregnant women and healthy men. The amount of hCG will vary greatly with gestational age and between individuals.
The NG-Test hCG WB has a sensitivity of 10mIU/mL and is capable of detecting pregnancy as early as 1 day before expected period date.
The addition of LH (300 mUI/mL), FSH (1000 mUI/mL) and TSH (1000 mUI/L) to negative (0 mIU/mL hCG) and positive (10 mIU/mL hCG) specimens showed no cross-reactivity.
The following potentially interfering substances were added to hCG negative and positive (10 mIU/mL) specimens.
Blood Pregnancy Test
You are now several days post ovulation and you suspect that this might be the cycle that you conceived! The next question that you might ask yourself is: should I run to the doctor for a blood pregnancy test or should I take a home pregnancy test?
Like most questions related to fertility and pregnancy, there is no straightforward answer to this question. Both a blood pregnancy test and a home pregnancy test will identify the presence of the pregnancy hormone called human chorionic gonadotropin (hCG), and will determine if you are pregnant or not. So, to determine which test is right for you, you might want to consider convenience, expense, accuracy, and your fertility history.
There are actually two different types of blood pregnancy tests, also called pregnancy serum tests. One type, called a quantitative blood pregnancy test (or a beta hCG test), measures the exact amount of hCG in the blood, while a qualitative blood pregnancy test simply provides confirmation of the presence of hCG, and a “yes” or “no” answer for pregnancy. If the blood pregnancy test reveals that hCG is under 5 mIU/ml, then the test will be considered negative. If the level of hCG is determined to be between 5 and 25 mIU/ml, this is said to be an equivocal result, and another test should be performed in a few days to confirm pregnancy.
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As for home pregnancy tests, there are several types to choose from. All home pregnancy tests (HPT) detect the presence of hCG in urine, and come either in a test strip, midstream or digital format. With the test strip or midstream format, you will dip the strip in a urine sample or urinate directly on the stick, wait a few minutes, and watch for the appearance of a colored test line to appear, which indicates a positive result. A digital test will typically provide a definitive “no” or “yes” in words that appear on the digital display. Each unique HPT is calibrated to detect a specific level of hCG in the urine, such as 20 mIU/ml, 50 mIU/ml or 100 mIU/ml. More “sensitive” tests, those that are able to detect a lower amount of hCG, can provide an accurate result as early as 7-10 days post ovulation.
When deciding if you should use an HPT or have a blood pregnancy test done at the lab, here are a few items to consider:
The following is a list of blood hCG levels achieved as pregnancy advances (LMP means last menstrual period):
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