Blood test for pregnancy in cattle
Cattle 28 DPB & 72 Days Post Calving – Cost: $2.75
Cattle (Embryo Transfer) 25 days post-implant or 32 days post-head – Cost: $2.75
Goat 30 Days Post- Breeding & 30 Days Post Buck Removal – Cost: $6.75 per Sample
The herd owner or vet follows these simple tasks:
– Makes a list of all eligible animals
– Assembles Vacuatiner® tubes (red or gray and red topped), needle holders, and needles (1 inch, 18 or 20 gauge) from a Bio Preg Check.
– Samples at least 2 cc (5 cc for wildlife) of blood
– Avoids cross contamination by using new supplies for each animal.
– Labels each tube using water-insoluble ink or pencil with the tube sequence # and the animal #.
– Packages samples for delivery to the laboratory
– Include a Sample Submittal Form with the sample and tube sequence numbers and list and fills in the blanks on the form.
The sample submittal form indicates how tests results are to be delivered (mail, fax, phone, e-mail).
Dr. Garth Sasser discovered pregnancy specific protein B (PSPB) from the placenta of cows. He developed a radioimmunoassay for the protein while he served as a professor at the University of Idaho, and was the first to show that a protein could be used as a blood-based test for pregnancy in ruminant animals.
BioTracking was established in 1992 for the development and marketing of a pregnancy-specific protein B (PSPB) test for pregnancy in cattle. The test was named BioPRYN ® , with PRYN standing for “Pregnant Ruminant Yes No”. This technology was licensed by BioTracking from the University of Idaho and the assay was converted to an enzyme-linked immunosorbent assay (ELISA). The result was BioPRYN, an affordable blood-based pregnancy test for cattle.
BioPRYN products for sheep and goats later became available, as did BioPRYNwild, a conversion of the radioimmunoassay (RIA) test to an ELISA. BioPRYNes, a test of equine blood for estrone sulfate was established in 2008 as a pregnancy test.
BioTracking, under the management of a team of skilled and knowledgeable staff, continues to build upon the tradition of rapid, friendly, and attentive service. The BioTracking research team is continuously working to make livestock pregnancy testing easier, more reliable and convenient, as the company strives for improved testing options that will benefit the livestock markets.
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BioTracking, Inc Business Model
BioTracking supplies BioPRYN testing kits to BioPRYN-licensed Affiliate Laboratories across the United States and internationally. These laboratories are independent of BioTracking but help make BioPRYN available to producers in many locations. A map showing the location of Affiliate Laboratories and methods for contacting them is available on the Lab Locator page.
Approximately 80 percent of all bovine BioPRYN tests are conducted by Affiliate Laboratories. Cattle, goat, sheep, equine, elk, deer and other wildlife tests are readily available at BioTracking, Inc in Moscow, Idaho.
Colorado State University
Researcher conceives new cow pregnancy test
Thomas “Tod” Hansen recently won the Animal Physiology and Endocrinology Award from the American Society of Animal Science.
As a young man working on his family ranch, Colorado State University reproductive scientist Thomas “Tod” Hansen checked cattle for pregnancy using conventional rectal palpation – a routine and inexpensive method that can be stressful for cows and physically demanding for technicians.
There’s got to be a better way, he thought.
Hansen, now director of the highly regarded CSU Animal Reproduction and Biotechnology Laboratory, has become a leading scientist working to understand the dynamics of livestock pregnancies at the molecular level. He also is leading new discoveries in the economically devastating bovine viral diarrhea virus (BVDV), in part because of links between cattle pregnancy and viral infection.
For these and other achievements, the American Society of Animal Science recently conferred to Hansen its Animal Physiology and Endocrinology Award for important research contributions to the livestock industry.
“Dr. Hansen has emerged as the leader in maternal recognition of pregnancy in ruminants,” CSU Distinguished Professor Emeritus George Seidel, an eminent reproductive scientist, wrote in an award-nomination letter. “His seminal discoveries have advanced our basic understanding of the mechanisms involved in embryo recognition by the mother, and also have provided new diagnostic tools to identify problems that limit reproductive efficiency.”
Hansen is developing a blood test to detect pregnancy in cattle as early as 16 days after breeding.
Hansen’s discoveries have led to several patents, critical steps in innovation and the introduction of new technologies to the marketplace. In this case, the patents were issued for blood markers that Hansen is using to develop pregnancy and viral screenings that would represent significant progress in managing livestock health.
“Our goal is to find the non-virally infected and non-pregnant animals in order to better manage reproduction and animal health,” Hansen explained.
He is developing a blood test to detect pregnancy in cattle as early as 16 days after breeding, compared to at least 25 days with ultrasound and some 35 days with rectal palpation. Such a test would detect pregnancy – or, more importantly, non-pregnancy – earlier than others on the market.
The time savings means a cow that is not pregnant could be quickly rebred so she remains productive and on pace with the rest of the herd, particularly important for purebred cattle operations and advanced dairies with thousands of milking cows. Considering there are more than 9 million dairy cows in the United States, the savings to producers would mount quickly.
Hansen also is investigating use of markers in new serum screenings that would identify cows and cattle fetuses infected with BVDV. The research is important in the context of reproduction because BVDV is transmitted from cow to unborn calf, resulting in aborted and stillborn calves, calves that die young, and calves that live but spread the virus to entire herds; the latter group, known as persistently infected, is the main source of BVDV infection in cattle.
Hansen studies livestock pregnancy and viral dynamics at a molecular level.
Such knowledge is important because BVDV is difficult to control: Vaccination programs are not universally implemented or 100 percent effective, and the virus spreads easily through the mouth or respiratory tract.
Much of Hansen’s work centers on interferon, a protein that signals pregnancy to a cow’s body and helps retain an embryo. He found that interferon triggers the creation of another protein, known as ISG15, a marker that could be central to identifying both pregnancy and viral infection.
“Subsequent research has demonstrated that ISG15 has important roles during pregnancy in all mammals studied to date, ranging from mice to humans,” Seidel wrote in his nomination letter, suggesting the far-reaching implications of Hansen’s discoveries.
A professor in the CSU Department of Biomedical Sciences, Hansen came to science through hands-on work on his family cattle ranch near Fort Collins. During calving season, he often referred to a CSU book titled, “Artificial Insemination and Reproductive Management of Cattle,” co-authored by Seidel, a member of the prestigious National Academy of Sciences and a pioneer in cattle reproduction.
“This book was like a bible to me,” Hansen recalled, reaching to an office shelf to retrieve his original, tattered copy.
He also was intrigued by embryo transfer and cryopreservation of gametes – advances brought to the family ranch by Hansen’s father, an obstetrician-gynecologist naturally interested in advances in reproductive science.
Seidel, who helped determine how to sort cattle sperm by gender, reflected on his colleague’s scientific honor.
“Tod has remarkable scientific expertise and success, as well as integrity and willingness to help others,” said Seidel, who is retired but still active at the CSU Animal Reproduction and Biotechnology Laboratory. “The award is for the science. The other is icing on the cake.”
Cattle pregnancy test
Very simple to perform test to determine a cow’s pregnancy quickly. With just one drop of blood, the veterinarian is able to test the animal for pregnancy directly in the barn – and that from just the 35th day after insemination!
The results of the cattle pregnancy test are available to the veterinarian after approx. 15 minutes and appear prominently on the test kit. This test detects the considerably more reliable PAG rather than progesterone.
- Cattle pregnancy rapid test
- Detection of PAG
- Test can be performed from the 35th day after insemination
- Very easy and quick – can be used directly in the barn
- Sample material: 1 drop of blood
- Test time: approx. 15 minutes
- Pack: 5 tests per pack (pipette is included)